Enzyme-catalyzed organic synthesis of sucrose and trehalose with in situ regeneration of UDP-glucose.
نویسندگان
چکیده
This paper describes cell-free enzymatic syntheses of sucrose and trehalose using partially-purified preparations of sucrose and trehalose synthetase. The coupling of the regeneration of uridine-5'-diphosphoglucose (UDP-Glc) with synthesis of the disaccharide offers a practical route to millimol quantities of these carbohydrates. The syntheses used pyruvate kinase, UDP-Glc pyrophosphorylase, and inorganic pyrophosphatase, and the regenerated UDP-Glc was cycled approximately 10 times.
منابع مشابه
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ورودعنوان ژورنال:
- Applied biochemistry and biotechnology
دوره 23 2 شماره
صفحات -
تاریخ انتشار 1990